c polyclonal goat anti type  (SouthernBiotech)

Journal: PLoS ONE

Article Title: Nuclear Factor Kappa-B Signaling Is Integral to Ocular Neovascularization in Ischemia-Independent Microenvironment

doi: 10.1371/journal.pone.0101602

Figure Lengend Snippet: The mRNA levels for the genes; NFκB/p65 , α5 , β1 , ET-1 , MMP-9 , FAK and EPO , were quantified by Real time RT-PCR. Selected experiments, which measured the mRNA levels of NFκB/p65 , indicated that YC-1 and SN50 downregulated the mRNA levels of NFκB/p65 as compared to DMSO or SN50M-treated retinas, respectively. For the other genes listed above, the mRNA levels were upregulated in the DMSO-treated retinas and the rho/VEGF group that was left untreated. In contrast, retinas from YC-1-treated retinas exhibited a significant downregulation of the mRNA expression as compared to retinas that were treated with DMSO. Despite the sustained expression of VEGF, there were no detectable differences in the levels of CXCR4 and SDF-1 mRNA expression in the animals of all groups. ANOVA was used for statistical analyses. Mean ± SEM of mRNA level normalized to β-actin were calculated, [*** P <0.001 and ** P <0.01, as compared to respective controls]. Data are representative of 3 independent experiments. J. Sequence for the Primer Sets Used for the Quantitative Real-Time PCR Analysis .

Article Snippet: Goat polyclonal anti-mouse C-X-C Chemokine Receptor Type 4 (CXCR4) antibody was obtained from LifeSpan BioSciences (Seattle, WA).

Techniques: Quantitative RT-PCR, Expressing, Sequencing, Real-time Polymerase Chain Reaction

Journal: PLoS ONE

Article Title: Nuclear Factor Kappa-B Signaling Is Integral to Ocular Neovascularization in Ischemia-Independent Microenvironment

doi: 10.1371/journal.pone.0101602

Figure Lengend Snippet: Immunohistochemical analysis of NFκB/p65, FAK, α5β1, EPO, ET-1, and MMP-9, has indicated the expression levels of these proteins were significantly elevated in the rho/VEGF retinas that were left untreated. YC-1-treated retinas exhibited a significant decrease in the protein expression levels as compared with DMSO-treated retinas. Despite the sustained expression of VEGF, there were no detectable differences in the levels of CXCR4 and SDF-1 protein expression amongst the animals of all groups. Retinas were examined at 100× objective. Scale bar, 100 µm.

Article Snippet: Goat polyclonal anti-mouse C-X-C Chemokine Receptor Type 4 (CXCR4) antibody was obtained from LifeSpan BioSciences (Seattle, WA).

Techniques: Immunohistochemical staining, Expressing

Journal: PLoS ONE

Article Title: Nuclear Factor Kappa-B Signaling Is Integral to Ocular Neovascularization in Ischemia-Independent Microenvironment

doi: 10.1371/journal.pone.0101602

Figure Lengend Snippet: Immunohistochemical analysis of NFκB/p65, FAK, α5β1, EPO, ET-1, and MMP-9, has indicated the expression levels of these proteins were significantly elevated in the rho/VEGF retinas that were left untreated. YC-1-treated retinas exhibited a significant decrease in the protein expression levels as compared with DMSO-treated retinas. Despite the sustained expression of VEGF, there were no detectable differences in the levels of CXCR4 and SDF-1 protein expression amongst the animals of all groups. Retinas were examined at 100× objective. Scale bar, 100 µm.

Article Snippet: Goat polyclonal anti-mouse C-X-C Chemokine Receptor Type 4 (CXCR4) antibody was obtained from LifeSpan BioSciences (Seattle, WA).

Techniques: Immunohistochemical staining, Expressing

Journal: PLoS ONE

Article Title: Nuclear Factor Kappa-B Signaling Is Integral to Ocular Neovascularization in Ischemia-Independent Microenvironment

doi: 10.1371/journal.pone.0101602

Figure Lengend Snippet: Immunohistochemical analysis of NFκB/p65, FAK, α5β1, EPO, ET-1, and MMP-9, has indicated the expression levels of these proteins were significantly elevated in the rho/VEGF retinas that were left untreated. YC-1-treated retinas exhibited a significant decrease in the protein expression levels as compared with DMSO-treated retinas. Despite the sustained expression of VEGF, there were no detectable differences in the levels of CXCR4 and SDF-1 protein expression amongst the animals of all groups. Retinas were examined at 100× objective. Scale bar, 100 µm.

Article Snippet: Goat polyclonal anti-mouse C-X-C Chemokine Receptor Type 4 (CXCR4) antibody was obtained from LifeSpan BioSciences (Seattle, WA).

Techniques: Immunohistochemical staining, Expressing

Journal: PLoS ONE

Article Title: Nuclear Factor Kappa-B Signaling Is Integral to Ocular Neovascularization in Ischemia-Independent Microenvironment

doi: 10.1371/journal.pone.0101602

Figure Lengend Snippet: Immunohistochemical analysis of NFκB/p65, FAK, α5β1, EPO, ET-1, and MMP-9, has indicated the expression levels of these proteins were significantly elevated in the rho/VEGF retinas that were left untreated. YC-1-treated retinas exhibited a significant decrease in the protein expression levels as compared with DMSO-treated retinas. Despite the sustained expression of VEGF, there were no detectable differences in the levels of CXCR4 and SDF-1 protein expression amongst the animals of all groups. Retinas were examined at 100× objective. Scale bar, 100 µm.

Article Snippet: Goat polyclonal anti-mouse C-X-C Chemokine Receptor Type 4 (CXCR4) antibody was obtained from LifeSpan BioSciences (Seattle, WA).

Techniques: Immunohistochemical staining, Expressing